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A novel leukocyte differentiation antigen: two monoclonal antibodies TM2
and TM3 define a 120-kd molecule present on neutrophils, monocytes,
platelets, and activated lymphoblasts
H Ohto, H Maeda, Y Shibata, RF Chen, Y Ozaki, M Higashihara, A Takeuchi and H Tohyama
We produced two hybridomas by fusion of mouse myeloma cells with
splenocytes from a mouse immunized with the THP-1 human monocytoid leukemia
cell line. Two cloned hybridoma cell lines, designated as TM2 and TM3, were
obtained. They secreted antibodies against a unique cell surface antigen
expressed on all normal peripheral blood monocytes, neutrophilic
granulocytes, platelets, and mitogen-induced lymphoblasts, some cells from
patients with immature-type lymphoid leukemias. However, the antibodies
reacted neither with large numbers of peripheral blood lymphocytes nor with
red cells. Cross-blocking studies showed that these monoclonal antibodies
recognized the same or a nearly positioned antigen epitope.
Immunoprecipitation of THP-1 cell extract with TM2 or TM3 under reducing
and nonreducing conditions yielded a specific band of mol wt equal to
120,000 daltons. This determinant appeared to be involved in granulocyte
chemotaxis, since neutrophilic granulocytes exposed to TM2 or TM3 showed a
significant decrease in chemotaxis toward endotoxin-activated serum. These
two monoclonal antibodies did not affect O2- release or luminol-dependent
chemiluminescence of neutrophils. Moreover, they did not alter platelet
aggregation induced by thrombin. TM2 and TM3 will provide a new reagent in
defining the linkage between lymphoid and myeloid differentiation and
intermyeloid development.
Volume 66,
Issue 4,
pp. 873-881,
10/01/1985
Copyright © 1985 by The American Society of Hematology
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