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Incidence of involvement of the B and T lymphocyte lineages in chronic
myelogenous leukemia
M Nitta, Y Kato, A Strife, M Wachter, J Fried, A Perez, S Jhanwar, R Duigou- Osterndorf, RS Chaganti and B Clarkson
Peripheral blood specimens were obtained from 22 patients with Philadelphia
chromosome (Ph1) positive chronic myelogenous leukemia (CML) (16 in chronic
phase, 2 in an accelerated phase, and 4 in blast crisis). Studies were
performed to determine the frequency of the presence of the Ph1 chromosome
in cells of lymphoid lineages. Rosetted (E+) lymphocytes (T lymphocytes)
from nine patients in chronic phase and one patient in blast crisis were
stimulated with T cell growth factor interleukin 2 (IL-2) and/or
phytohemagglutinin (PHA). All ten patients had sufficient T lymphocyte
metaphases for analysis and of a total of 461 metaphases examined, only one
contained the Ph1 chromosome. Nucleated cells of density less than 1.077
g/mL were infected with Epstein-Barr virus (EBV). Following infection, cell
lines were established from individual colonies attached to egg albumin-
coated Lab-Tek slide chambers (clonal cell lines) or from suspension
culture in 96-well tissue culture cluster dishes (nonclonal cell lines).
Cell surface and intracellular marker analysis confirmed the B lymphocyte
phenotype of all the cell lines examined. B lymphoblastoid cell lines were
established from 16 of the 22 patients. All lines from 12 patients were
Ph1-negative. From two chronic phase patients, both Ph1-positive and
Ph1-negative lines were established. From one patient in an accelerated
phase, only Ph1-positive lines were established. From another patient in
blast crisis (of myeloblastic phenotype), only Ph1- positive lines were
established initially; however, five months later, after the patient had
been treated with mitoxantrone, only Ph1-negative lines were derived from
this patient. Based on these results, it appears that most B cells and
mature T cells in most CML patients are Ph1-negative, but that about 25% of
patients have predominantly Ph1- positive B cells or a mixture of
Ph1-positive and Ph1-negative B cells that are capable of growing as
established cell lines after transformation with EBV.
Volume 66,
Issue 5,
pp. 1053-1061,
11/01/1985
Copyright © 1985 by The American Society of Hematology
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