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FT Slovick, CN Abboud, JK Brennan and MA Lichtman
The growth of human eosinophil progenitors (CFU-Eo) and the modulation of
growth by hydrocortisone were studied as functions of the presence of
lymphocytes and monocytes in marrow cells under study; and the source of
colony-stimulating factors, specifically, media conditioned by
macrophage-like cell line, GCT; phytohemagglutinin-stimulated mononuclear
cells (PHA-LCM); or the T cell line, MO. CFU-Eo growth was greatest in
marrow containing accessory cells as compared to marrow depleted of
accessory cells; and in marrow treated with phytohemagglutinin-stimulated
leukocyte conditioned media (PHA-LCM) or MO (T cell line)-conditioned
medium (MO-CM) as compared with GCT cell- conditioned medium (GCT-CM).
Hydrocortisone reproducibly inhibited eosinophil progenitor growth in
unfractionated marrow stimulated by GCT- CM. This effect was abrogated by
admixing irradiated mononuclear cells or T lymphocytes with the target
marrow or by adding interleukin 1 or interleukin 2 (IL-1, IL-2). Inhibition
by hydrocortisone did not occur when monocyte and T lymphocyte depleted
marrow was studied. Unlike GCT- CM, MO-CM and PHA-LCM stimulated equal
proportions of eosinophil progenitors in nondepleted and accessory
cell-depleted marrow and demonstrated less hydrocortisone inhibition.
However, both GCT-CM and PHA-LCM produced in the presence of hydrocortisone
stimulated significantly fewer CFU-Eos in both unfractionated and accessory
cell- depleted marrow target populations. These results indicate that the
growth of CFU-Eo and inhibition of growth by hydrocortisone is a direct
function of a monocyte-T cell interaction and probably is mediated through
effects on the production/release of eosinophil colony stimulating factor
(Eo-CSF).
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| Copyright © 1985 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
