CGA-7 and HHF, two monoclonal antibodies that recognize muscle actin and
react with adherent cells in human long-term bone marrow cultures
P Charbord, AM Gown, A Keating and JW Singer
The CGA-7, a monoclonal antibody that reacts with smooth muscle cell actin
but not with endothelial cell or fibroblast actin, and HHF, a monoclonal
antibody that reacts with smooth muscle, skeletal muscle, and cardiac
muscle actin, both recognize microfilaments present within adherent cells
from actively hematopoietic human long-term marrow cultures. Macrophages,
monocytes, and cultured marrow fibroblasts do not react with either
antibody. These data suggest that the anti-actin antibodies may serve as
useful markers for in vitro microenvironmental cells and lend support to
the hypothesis that stromal cells from long- term marrow cultures are
different from marrow fibroblasts and may constitute a unique cell lineage.
Volume 66,
Issue 5,
pp. 1138-1142,
11/01/1985
Copyright © 1985 by The American Society of Hematology
