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M Allouche, A Bourinbaiar, V Georgoulias, R Consolini, A Salvatore, H Auclair and C Jasmin
Cytochemical and immunologic analysis of cells obtained from two patients
with chronic myeloid leukemia (CML) during blast crisis reveals markers
suggestive of an immature lymphoid phenotype. Peripheral blood mononuclear
cells from both patients generated spontaneous lymphoblastoid colonies in
methylcellulose, a phenomenon observed in T cell acute lymphoblastic
leukemias and T cell non- Hodgkin's lymphomas but not in any other type of
leukemia. Colonies derived from one patient were composed predominantly of
OKT3+ cells (89%), whereas those from the second patient displayed 42%
OKT3+ and OKT6+ cells. In the second patient's colonies, each of five
mitoses contained the Philadelphia chromosome (Ph1) and two of five
displayed the same additional karyotypic abnormalities as the blast crisis
cells. Cells obtained from the two patients during remission still gave
rise to spontaneous T cell colonies (greater than 85% OKT3+) and Ph1 was
detected in 33% and 60% of the metaphases, respectively. However, when
colony growth was induced by an interleukin 2-containing conditioned
medium, less than 5% of mitoses were Ph1-positive. These data suggest that:
(1) the T cell lineage might be involved in CML; (2) a subset of T cells
may remain unaffected by the leukemic process, as demonstrated by the
virtual absence of Ph1 in induced T cell colonies; and (3) the spontaneous
colony assay seems to select for the growth of malignant T cells.
This article has been cited by other articles:
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| Copyright © 1985 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
