Platelet-derived growth factor does not stimulate prostacyclin synthesis by
cultured endothelial cells
KS Callahan, A Schorer and JM Harlan
We examined the effect of highly purified platelet-derived growth factor
(PDGF) on prostacyclin (PGI2) release by cultured human umbilical vein and
bovine aortic endothelial cells. PDGF tested at concentrations equal to or
exceeding those observed in serum did not increase endothelial cell PGI2
synthesis as measured by radioimmunoassay of its metabolite, 6-keto-PGF1
alpha. In contrast, cells incubated with 20% human whole blood serum (WBS)
demonstrated significantly increased PGI2 production (fivefold
stimulation). Addition of anti-PDGF antibody to the 20% WBS did not
attenuate the increased synthesis of PGI2. Incubation with 20%
plasma-derived serum (PDS) that was deficient in PDGF produced stimulation
of PGI2 release similar to 20% WBS. These results demonstrate that PDGF
does not cause increased PGI2 synthesis in cultured human endothelial cells
of human or bovine origin, and further suggest that the stimulation
observed with serum is not due to a platelet-release product.
Volume 67,
Issue 1,
pp. 131-134,
01/01/1986
Copyright © 1986 by The American Society of Hematology
