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Chronic B-lymphocytic leukemia cells proliferate and differentiate
following exposure to interferon in vitro
L Ostlund, S Einhorn, KH Robert, G Juliusson and P Biberfeld
The ability of interferon (IFN) to induce proliferation and differentiation
in malignant B cells from 29 patients with chronic lymphocytic leukemia
(CLL) and in lymphoid cells from 11 healthy donors was investigated. IFN
induced transformation and plasmacytoid differentiation in B cells from 19
of 29 CLL patients. The transformed cells belonged to the malignant clone
as indicated by the immunoglobulin (lg) light chain restriction. Cells
exposed to IFN expressed intracellular lg to a varying degree, which was
correlated to the level of plasmacytoid differentiation. IFN gave rise to
proliferative responses in cells from three patients. Cytogenetic studies
on lymphoid cells from one patient showed that proliferation occurred in
the malignant B cells. Induction of proliferation and differentiation was
observed with various alpha-IFN and gamma-IFN preparations, as well as with
a completely pure beta-IFN, showing that IFN and not contaminants in the
preparations were responsible for the observed effects. Maximal
transformation and proliferation usually occurred after four days of
incubation at an IFN concentration of 500 to 5,000 U/mL. The ability of IFN
to induce differentiation in CLL cells may be of importance for the
reported antitumoral effects observed in some B cell malignancies during
IFN therapy.
Volume 67,
Issue 1,
pp. 152-159,
01/01/1986
Copyright © 1986 by The American Society of Hematology
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