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Biologic properties in vitro of a recombinant human granulocyte- macrophage
colony-stimulating factor
D Metcalf, CG Begley, GR Johnson, NA Nicola, MA Vadas, AF Lopez, DJ Williamson, GG Wong, SC Clark and EA Wang
Recombinant human granulocyte-macrophage colony-stimulating factor (rH
GM-CSF) was purified to homogeneity from medium conditioned by COS cells
transfected with a cloned human GM-CSF cDNA and shown to be an effective
proliferative stimulus in human marrow cultures for GM and eosinophil
colony formation. The specific activity of purified rH GM- CSF in human
marrow cultures was calculated to be at least 4 X 10(7) U/mg protein. Clone
transfer experiments showed that this proliferation was due to direct
stimulation of responding clonogenic cells. Acting alone, rH GM-CSF did not
stimulate erythroid colony formation, but in combination with
erythropoietin, increased erythroid and multipotential colony formation in
cultures of peripheral blood cells. rH GM-CSF had no proliferative effects
on adult or fetal murine hematopoietic cells, did not induce
differentiation in murine myelomonocytic WEHI-3B cells, and was unable to
stimulate the survival or proliferation of murine hematopoietic cell lines
dependent on murine multi-CSF (IL 3). rH GM- CSF stimulated
antibody-dependent cytolysis of tumor cells by both mature human
neutrophils and eosinophils and increased eosinophil autofluorescence and
phagocytosis by neutrophils. From a comparison of these effects with those
of semipurified preparations of human CSF alpha and -beta, it was concluded
that rH GM-CSF exhibited all the biologic activities previously noted for
CSF alpha.
Volume 67,
Issue 1,
pp. 37-45,
01/01/1986
Copyright © 1986 by The American Society of Hematology
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