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A monoclonal antibody against an erythrocyte ontogenic antigen identifies
fetal and adult erythroid progenitors
P Edelman, G Vinci, JL Villeval, W Vainchenker, A Henri, R Miglierina, P Rouger, J Reviron, J Breton-Gorius and C Sureau
A murine monoclonal antibody (MoAb) designated FA6-152 has been obtained by
immunizing mice with fetal erythrocytes. This antibody agglutinates fetal
but not adult erythrocytes. Among blood cells, this antibody bound to both
adult and fetal monocytes, platelets, and reticulocytes, but did not react
with lymphocytes and granulocytes. Fluorescent labeling of marrow cells and
of in vitro BFU-E, CFU-GM, and CFU-MK-derived colonies has shown that the
antigen defined by FA6-152 MoAb was absent from the granulocytic precursors
and was detected on the megakaryocytic lineage at a later stage of
differentiation than the platelet-specific markers. In contrast, the
antigen appeared as a very early marker of the erythroid differentiation
since all erythroblasts, including proerythroblasts, were labeled even
before the expression of glycophorin A. Cells from adult marrow and fetal
liver were sorted with the FA6-152 MoAb and studied by electron microscopy
and cell culture. The negative fraction contained granulocytic, monocytic,
and megakaryocytic precursors, whereas the positive fraction was devoid of
these precursors and contained monocytes, erythroblasts at all stages of
maturation, and a homogeneous population of blasts. Cultures have shown
that the only hematopoietic progenitors present in this positive fraction
were CFU-E and some BFU-E. The antigenic density was related to the
differentiation stage of the erythroid progenitors. In conclusion, this
antibody is similar to the previously described 5F1 MoAb (Bernstein and
Andrews, J Immunol 128:876, 1982; and Andrews et al, Blood 62:124, 1983)
and provides a useful probe for studies leading to improved understanding
of normal and malignant erythroid differentiation.
Volume 67,
Issue 1,
pp. 56-63,
01/01/1986
Copyright © 1986 by The American Society of Hematology
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