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Immunohistochemical localization of membrane and alpha-granule proteins in
human megakaryocytes: application to plastic-embedded bone marrow biopsy
specimens
JH Beckstead, PE Stenberg, RP McEver, MA Shuman and DF Bainton
Using a new technique for antigen localization, we have demonstrated
platelet proteins in megakaryocytes in plastic-embedded biopsy specimens of
normal human bone marrow. In a series of 25 specimens, megakaryocytes
showed labeling with antibodies to the integral membrane glycoproteins
IIIa, IIb, and the IIb-IIIa complex; granule membrane protein 140; and five
alpha-granule matrix proteins: thrombospondin, factor VIII-related antigen,
beta-thromboglobulin, platelet factor 4, and fibrinogen. The antibodies to
the membrane glycoproteins IIIa, IIb, and IIb-IIIa produced diffuse
cytoplasmic staining and heavier staining on the plasma membrane, whereas
the antibodies to the alpha-granule matrix proteins produced a distinct
granular staining within the cytoplasm. Staining for granule membrane
protein 140 was also granular in distribution. Rare mononuclear cells
consistent with megakaryocyte precursors were labeled with these markers.
Other enzyme histochemical and lectin-binding studies showed that the
enzyme alpha-naphthyl acetate esterase, the lectin Ulex europaeus I, and
the periodic-acid Schiff reaction were consistent, but not specific,
markers of megakaryocytes. This immunohistochemical technique should
facilitate the examination of qualitative and quantitative changes in
megakaryocytes in a variety of physiologic and pathologic processes.
Volume 67,
Issue 2,
pp. 285-293,
02/01/1986
Copyright © 1986 by The American Society of Hematology
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