|
|
 Previous Article | Table of Contents | Next Article 
Platelet adhesion and thrombus formation on subendothelium in platelets
deficient in glycoproteins IIb-IIIa, Ib, and storage granules
HJ Weiss, VT Turitto and HR Baumgartner
Patients whose platelets are deficient in glycoprotein (GP) Ib, IIb- IIIa
(thrombasthenia), or granule substances (storage pool deficiency, SPD) were
studied to define further the properties of platelets that mediate platelet
adhesion and thrombus formation on subendothelium. Both nonanticoagulated
and citrated blood were exposed to everted, de- endothelialized rabbit
vessel segments under controlled flow conditions and shear rates varying
from 650 to 3,300 sec-1. Morphometry was used to measure platelet thrombus
dimensions and the percentage of the subendothelial surface covered with
contact (C) or spread (S) platelets. Adhesion was defined as C + S. The
results in SPD demonstrated (1) reduced thrombus dimensions in delta-SPD
(pure dense granule deficiency) in proportion to the magnitude of the dense
granule defect; (2) an even greater reduction in thrombus dimensions in
patients with combined deficiencies of alpha and dense granules (alpha
delta-SPD); and (3) impaired platelet adhesion at several conditions in
alpha delta-SPD and, in delta-SPD, a hematocrit-dependent impairment of
adhesion in citrated blood at 2,600 sec-1. In thrombasthenia, platelets
were present as a monolayer on the subendothelial surface in both
nonanticoagulated and citrated blood, indicating an absolute requirement
for GPIIb-IIIa in promoting platelet-platelet interaction at all shear
rates and perfusion times. Two types of abnormalities in platelet-vessel
wall interactions were observed. In nonanticoagulated blood, the percentage
of platelets in the C phase was consistently increased at all shear rates,
but C + S values were normal. These observations indicate that platelets
deficient in GPIIb-IIIa do not spread normally on the subendothelial
surface exposed to nonanticoagulated blood. With citrated blood, the C + S
value in thrombasthenia was reduced at both 800 and 2,600 sec-1, as in von
Willebrand's disease, and a similar degree of reduction (about 50%) was
observed in normal blood treated with a monoclonal antibody to GPIIb- IIIa.
The findings, together with theoretical considerations, are consistent with
an hypothesis that GPIIb-IIIa mediates the spreading of platelets on
subendothelium following the initial attachment through GPIb and that
GPIIb-IIIa may be considered an adhesion site on the platelet membrane.
Abnormalities of GPIIb-IIIa may, depending on the conditions of study,
result in either increased values of C platelets or decreased values of C +
S. The results of the study further suggest that a complex interaction of
platelet granule factors and membrane GP mediate platelet adhesion and
thrombus formation.
Volume 67,
Issue 2,
pp. 322-330,
02/01/1986
Copyright © 1986 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. J. Graham, Q. Ren, J. R. Dilks, P. Blair, S. W. Whiteheart, and R. Flaumenhaft
Endobrevin/VAMP-8-dependent dense granule release mediates thrombus formation in vivo
Blood,
July 30, 2009;
114(5):
1083 - 1090.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Urbanus, R. Derksen, and P. de Groot
Platelets and the antiphospholipid syndrome
Lupus,
October 1, 2008;
17(10):
888 - 894.
[Abstract]
[PDF]
|
|
|
|
|
|
|
C. Strassel, C. Nonne, A. Eckly, T. David, C. Leon, M. Freund, J.-P. Cazenave, C. Gachet, and F. Lanza
Decreased Thrombotic Tendency in Mouse Models of the Bernard-Soulier Syndrome
Arterioscler Thromb Vasc Biol,
January 1, 2007;
27(1):
241 - 247.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Resendiz, S. Feng, G. Ji, K. A. Francis, M. C. Berndt, and M. H. Kroll
Purinergic P2Y12 Receptor Blockade Inhibits Shear-Induced Platelet Phosphatidylinositol 3-Kinase Activation
Mol. Pharmacol.,
March 1, 2003;
63(3):
639 - 645.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Remijn, Y.-P. Wu, E. H. Jeninga, M. J.W. IJsseldijk, G. van Willigen, P. G. de Groot, J. J. Sixma, A. T. Nurden, and P. Nurden
Role of ADP Receptor P2Y12 in Platelet Adhesion and Thrombus Formation in Flowing Blood
Arterioscler Thromb Vasc Biol,
April 1, 2002;
22(4):
686 - 691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. W. de Rossi, N. A. Horn, J. H. Baumert, K. Gutensohn, G. Hutschenreuter, and R. Rossaint
Xenon Does Not Affect Human Platelet Function In Vitro
Anesth. Analg.,
September 1, 2001;
93(3):
635 - 640.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-P. Wu, T. Vink, M. Schiphorst, G. H. van Zanten, M. J. W. IJsseldijk, P. G. de Groot, and J. J. Sixma
Platelet Thrombus Formation on Collagen at High Shear Rates Is Mediated by von Willebrand Factor-Glycoprotein Ib Interaction and Inhibited by von Willebrand Factor-Glycoprotein IIb/IIIa Interaction
Arterioscler Thromb Vasc Biol,
June 1, 2000;
20(6):
1661 - 1667.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Zaffran, S. C. Meyer, E. Negrescu, K. B. Reddy, and J. E. B. Fox
Signaling across the Platelet Adhesion Receptor Glycoprotein Ib-IX Induces alpha IIbbeta 3 Activation Both in Platelets and a Transfected Chinese Hamster Ovary Cell System
J. Biol. Chem.,
May 26, 2000;
275(22):
16779 - 16787.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. J. Fredrickson, N. A. Turner, N. S. Kleiman, N. Graziadei, K. Maresh, M. A. Mascelli, M. B. Effron, and L. V. McIntire
Effects of Abciximab, Ticlopidine, and Combined Abciximab/Ticlopidine Therapy on Platelet and Leukocyte Function in Patients Undergoing Coronary Angioplasty
Circulation,
March 14, 2000;
101(10):
1122 - 1129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Yuan, S. Kulkarni, P. Ulsemer, S. L. Cranmer, C. L. Yap, W. S. Nesbitt, I. Harper, N. Mistry, S. M. Dopheide, S. C. Hughan, et al.
The von Willebrand Factor-Glycoprotein Ib/V/IX Interaction Induces Actin Polymerization and Cytoskeletal Reorganization in Rolling Platelets and Glycoprotein Ib/V/IX-transfected Cells
J. Biol. Chem.,
December 17, 1999;
274(51):
36241 - 36251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. White, M. D. Krumwiede, and G. Escolar
Glycoprotein Ib Is Homogeneously Distributed on External and Internal Membranes of Resting Platelets
Am. J. Pathol.,
December 1, 1999;
155(6):
2127 - 2134.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gu, X. Xi, G. D. Englund, M. C. Berndt, and X. Du
Analysis of the Roles of 14-3-3 in the Platelet Glycoprotein Ib-IX-Mediated Activation of Integrin {alpha}IIb{beta}3 Using a Reconstituted Mammalian Cell Expression Model
J. Cell Biol.,
November 29, 1999;
147(5):
1085 - 1096.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Bodnar, M. Gu, Z. Li, G. D. Englund, and X. Du
The Cytoplasmic Domain of the Platelet Glycoprotein Ibalpha Is Phosphorylated at Serine 609
J. Biol. Chem.,
November 19, 1999;
274(47):
33474 - 33479.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tsuji, M. Sugimoto, S. Miyata, M. Kuwahara, S. Kinoshita, and A. Yoshioka
Real-Time Analysis of Mural Thrombus Formation in Various Platelet Aggregation Disorders: Distinct Shear-Dependent Roles of Platelet Receptors and Adhesive Proteins Under Flow
Blood,
August 1, 1999;
94(3):
968 - 975.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Zurbano, I. Anguera, M. Heras, E. Roig, M. Lozano, G. Sanz, and G. Escolar
Captopril Administration Reduces Thrombus Formation and Surface Expression of Platelet Glycoprotein IIb/IIIa in Early Postmyocardial Infarction Stage
Arterioscler Thromb Vasc Biol,
July 1, 1999;
19(7):
1791 - 1795.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. M. Ruggeri, J. A. Dent, and E. Saldivar
Contribution of Distinct Adhesive Interactions to Platelet Aggregation in Flowing Blood
Blood,
July 1, 1999;
94(1):
172 - 178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. J. Fredrickson, J.-F. Dong, L. V. McIntire, and J. A. Lopez
Shear-Dependent Rolling on von Willebrand Factor of Mammalian Cells Expressing the Platelet Glycoprotein Ib-IX-V Complex
Blood,
November 15, 1998;
92(10):
3684 - 3693.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Aplin, A. Howe, S. K. Alahari, and R. L. Juliano
Signal Transduction and Signal Modulation by Cell Adhesion Receptors: The Role of Integrins, Cadherins, Immunoglobulin-Cell Adhesion Molecules, and Selectins
Pharmacol. Rev.,
June 1, 1998;
50(2):
197 - 264.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. M. Grimaldi, F. Chen, C. Wu, H. J. Weiss, B. S. Coller, and D. L. French
Glycoprotein IIb Leu214Pro Mutation Produces Glanzmann Thrombasthenia With Both Quantitative and Qualitative Abnormalities in GPIIb/IIIa
Blood,
March 1, 1998;
91(5):
1562 - 1571.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Galbusera, C. Zoja, R. Donadelli, S. Paris, M. Morigi, A. Benigni, M. Figliuzzi, G. Remuzzi, and A. Remuzzi
Fluid Shear Stress Modulates von Willebrand Factor Release From Human Vascular Endothelium
Blood,
August 15, 1997;
90(4):
1558 - 1564.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Gruel, G. Pacouret, S. Bellucci, and J. Caen
Severe Proximal Deep Vein Thrombosis in a Glanzmann Thrombasthenia Variant Successfully Treated With a Low Molecular Weight Heparin
Blood,
July 15, 1997;
90(2):
888 - 889.
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. A. Hagberg, H. E. Roald, and T. Lyberg
Platelet Activation in Flowing Blood Passing Growing Arterial Thrombi
Arterioscler Thromb Vasc Biol,
July 1, 1997;
17(7):
1331 - 1336.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
P. Andre, P. Hainaud, C. Bal dit Sollier, L. I. Garfinkel, J. P. Caen, and L. O. Drouet
Relative Involvement of GPIb/IX-vWF Axis and GPIIb/IIIa in Thrombus Growth at High Shear Rates in the Guinea Pig
Arterioscler Thromb Vasc Biol,
May 1, 1997;
17(5):
919 - 924.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
N. J. Greco
Functional Expression of a P2T ADP Receptor in Xenopus Oocytes Injected With Megakaryocyte (CMK 11-5) RNA
Arterioscler Thromb Vasc Biol,
April 1, 1997;
17(4):
769 - 777.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
H. J. Weiss and B. Lages
Platelet Prothrombinase Activity and Intracellular Calcium Responses in Patients With Storage Pool Deficiency, Glycoprotein IIb-IIIa Deficiency, or Impaired Platelet Coagulant Activity --- A Comparison With Scott Syndrome
Blood,
March 1, 1997;
89(5):
1599 - 1611.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.G. White, M.D. Krumwiede, D.J. Cocking-Johnson, and G. Escolar
Uptake of vWF–Anti-vWF Complexes by Platelets in Suspension
Arterioscler Thromb Vasc Biol,
July 1, 1996;
16(7):
868 - 877.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. G. Cunningham, S. C. Meyer, and J. E. B. Fox
The Cytoplasmic Domain of the alpha-Subunit of Glycoprotein (GP) Ib Mediates Attachment of the Entire GP Ib-IX Complex to the Cytoskeleton and Regulates von Willebrand Factor-induced Changes in Cell Morphology
J. Biol. Chem.,
May 10, 1996;
271(19):
11581 - 11587.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Andre, B. Arbeille, V. Drouet, P. Hainaud, C. Bal dit Sollier, J. P. Caen, and L. O. Drouet
Optimal Antagonism of GPIIb/IIIa Favors Platelet Adhesion by Inhibiting Thrombus Growth : An Ex Vivo Capillary Perfusion Chamber Study in the Guinea Pig
Arterioscler Thromb Vasc Biol,
January 1, 1996;
16(1):
56 - 63.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
C. L. Yap, S. C. Hughan, S. L. Cranmer, W. S. Nesbitt, M. M. Rooney, S. Giuliano, S. Kulkarni, S. M. Dopheide, Y. Yuan, H. H. Salem, et al.
Synergistic Adhesive Interactions and Signaling Mechanisms Operating between Platelet Glycoprotein Ib/IX and Integrin alpha IIbbeta 3. STUDIES IN HUMAN PLATELETS AND TRANSFECTED CHINESE HAMSTER OVARY CELLS
J. Biol. Chem.,
December 22, 2000;
275(52):
41377 - 41388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Remijn, Y.-P. Wu, E. H. Jeninga, M. J.W. IJsseldijk, G. van Willigen, P. G. de Groot, J. J. Sixma, A. T. Nurden, and P. Nurden
Role of ADP Receptor P2Y12 in Platelet Adhesion and Thrombus Formation in Flowing Blood
Arterioscler Thromb Vasc Biol,
April 1, 2002;
22(4):
686 - 691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
