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Platelet-leukocyte interaction: selective binding of thrombin- stimulated
platelets to human monocytes, polymorphonuclear leukocytes, and related
cell lines
TW Jungi, MO Spycher, UE Nydegger and S Barandun
The association of platelets with leukocytes was investigated, using
gel-filtered platelets stimulated with thrombin and then fixed with
formaldehyde. Evidence is presented that stimulation of gel-filtered
platelets with low concentrations of thrombin (0.01 to 0.1 U/mL) induces
the expression of surface determinants interacting strongly with monocytes
and polymorphonuclear leukocytes (PMNs) but only weakly with lymphocytes.
Both monocyte-platelet binding and PMN-platelet binding occurred at 37
degrees C and more intensively at 0 degrees C; it was Ca2+-dependent and
was unaffected by the addition of sodium azide. The binding also occurred
with the monocytoid cell lines HL 60 and U 937 in exponential growth and
was much less two days after induction of terminal differentiation by
phorbol myristate acetate (PMA). No other tested cell lines (B cells, T
cells, and myeloid cells) bound thrombin-stimulated platelets.
Monocyte-derived macrophages kept in culture for one week also exhibited
reduced binding of thrombin- stimulated platelets. IgG and fibronectin
could be ruled out as ligands that mediate binding.
Volume 67,
Issue 3,
pp. 629-636,
03/01/1986
Copyright © 1986 by The American Society of Hematology
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