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Cytogenetic and immunophenotypic analysis of cell lines established from
patients with T cell leukemia/lymphoma
SD Smith, R Morgan, MP Link, P McFall and F Hecht
Cell lines were established from five patients with T cell malignancies.
Two patients had T cell lymphoblastic lymphoma (T-LL), whereas three
patients had T cell acute lymphoblastic leukemia (T-ALL). Both T-LL cell
lines expressed cell surface antigens characteristic of midthymocytes (Leu
2, 3, 6+). One T-ALL cell line also expressed this immunophenotype, one
expressed suppressor/cytotoxic antigens (Leu 2+; Leu 3, 6-), and one
expressed antigens of a mature but uncommitted T cell (Leu 4+; Leu 2, 3,
6-). Cytogenetic analysis showed that each cell line had 46 chromosomes
with pseudodiploidy. The three T-ALL cell lines had only a few chromosome
changes; one cell line had one deletion, another had two deletions, and the
third had a translocation and two deletions (including loss of part of 9p).
In comparison, both T-LL cell lines had complex chromosome changes,
including most notably a rearrangement of band 14q11.2. The
immunophenotypes and chromosome breakpoints showed patterns of interlock
between the T-LL and T-ALL cell lines because common abnormalities occurred
at six distinct chromosome sites. Cell lines with limited and specific
chromosomal abnormalities are important because they can provide the basic
material for molecular genetic studies that could elucidate the genetic
mechanisms involved in neoplasia.
Volume 67,
Issue 3,
pp. 650-656,
03/01/1986
Copyright © 1986 by The American Society of Hematology
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