Macrophage colony formation supported by purified CSF-1 and/or interleukin
3 in serum-free culture: evidence for hierarchical difference in macrophage
colony-forming cells
K Koike, ER Stanley, JN Ihle and M Ogawa
Using a serum-free culture system, we examined murine macrophage colony
formation from bone marrow cells cultured in the presence of purified
CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1
supported macrophage and neutrophil-macrophage colony formation, whereas
IL-3 supported the formation of various types of single lineage and
multilineage colonies. CSF-1 supported more macrophage colonies from bone
marrow cells of normal mice than IL 3, whereas in cultures of bone marrow
cells of 5-fluorouracil-treated mice, IL 3 supported more macrophage
colonies. A combination of CSF-1 and IL 3 resulted in
granulocyte-macrophage (GM) colony formation that was equal to or greater
than the sum of GM colony formation supported by the factors individually.
The combination of CSF-1 and IL 3 resulted in significant increases in the
size of both macrophage and neutrophil-macrophage colonies. Similar
increases in colony size were observed when CSF-1 was added to cultures
five days after incubation of marrow cells with IL 3. These data support
the concept that some of the macrophage colony- forming cells that respond
to IL 3 are more primitive than those that are sensitive to CSF-1.
Volume 67,
Issue 4,
pp. 859-864,
04/01/1986
Copyright © 1986 by The American Society of Hematology
