An explanation for nonimmunologic adsorption of proteins onto red blood
cells: Schiff's base reactions
D Jamin, J Demers, I Shulman, HT Lam and R Momparler
During a phase I evaluation of diglycoaldehyde (INOX), an intravenous
chemotherapeutic agent used to treat children with malignancies, all of
eight patients tested developed a positive direct antiglobulin test (DAT)
in vivo. The DAT became positive within one to seven days after the first
administration of the drug and remained positive for up to 12 days
following the last dose. The indirect antiglobulin tests were negative.
None of the patients showed clinical or laboratory evidence of hemolysis at
the time the DAT became positive or during follow-up. Eluates made from the
red cells of two of the eight patients were both negative by indirect
antihuman globulin testing. In vitro studies with INOX and glutaraldehyde,
both dialdehyde compounds, showed nonimmunologic adsorption of protein onto
red cells, probably by the condensation of aldehyde groups of these
compounds to form Schiff's bases with amino acids of serum proteins and red
cell membrane proteins. This reaction provides an explanation for the
globulin detected on the red cells of patients treated with INOX.
Volume 67,
Issue 4,
pp. 993-996,
04/01/1986
Copyright © 1986 by The American Society of Hematology
