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Synchronized cultures of P falciparum in abnormal red cells: the mechanism
of the inhibition of growth in HbCC cells
JA Olson and RL Nagel
It has been previously demonstrated that HbCC cells fail to support growth
of P falciparum in asynchronous Jensen-Trager cultures. To define the
mechanism of inhibition we have studied synchronous cultures and found that
while intraerythrocytic parasite development appeared normal, the
liberation of merozoites and/or invasion was impaired. This effect was
detected by a normal growth during the first growth cycle but dramatically
reduced number of ring forms following the schizont stage. A specific test
for the invasion of CC cells by P falciparum merozoites, nevertheless, was
normal. The defect found in infected CC cells was not modified by changes
in O2 tension (which altered the ligand saturation of Hb C) nor by the
extracellular K+ concentration (excluding a K+ leak-dependent mechanism for
the growth inhibition). The osmotic lysis of late-staged parasitized red
cells revealed that 25% of infected AA cells were lysed when the
extracellular medium was 95 mOsm. In contrast, infected CC cells required a
decrease to 10 mOsm in the extracellular media to reach 25% lysis. We
conclude that CC red cells are unsuitable hosts for the malarial parasite
primarily because of their inability to lyse and release merozoites at the
appropriate stage of intraerythrocytic development of P falciparum.
Volume 67,
Issue 4,
pp. 997-1001,
04/01/1986
Copyright © 1986 by The American Society of Hematology
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