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This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stricker, R. B. Articles by Shuman, M. A. Search for Related Content PubMed PubMed Citation Articles by Stricker, R. B. Articles by Shuman, M. A. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Quinidine purpura: evidence that glycoprotein V is a target platelet antigen RB Stricker and MA Shuman Quinidine-induced thrombocytopenia has been associated with both immune complex and autoantibody binding to platelets. In the present study, serum antibody from six of six patients with quindine purpura was shown by immunoblotting to bind to a single platelet membrane protein of mol wt 80,000. This target protein was absent from Bernard-Soulier (BSS) platelets. F(ab)2 prepared from one patient's serum also bound to this protein, indicating autoantibody rather than immune complex binding to the target antigen. Antibody binding to the 80-kd protein was preserved after treatment of platelets with concentrations of trypsin or chymotrypsin that completely removed glycoprotein Ib (GPIb). Preincubation of platelet proteins with one patient's serum blocked binding of a polyclonal rabbit antibody against glycoprotein V (GPV), indicating that these antibodies recognize the same antigen. By wheat germ affinity chromatography, GPV was shown to copurify with GPIb. Quinidine-induced antibody bound to the wheat germ-purified GPV but not to GPIb. We conclude that quinidine purpura is associated with autoantibody directed against platelet GPV. Volume 67, Issue 5, pp. 1377-1381, 05/01/1986 Copyright © 1986 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. G. Wittels, R. D. Siegel, and E. M. Mazur Thrombocytopenia in the Intensive Care Unit Setting J Intensive Care Med, September 1, 1990; 5(5): 224 - 240. [Abstract] [PDF]

	Copyright © 1986 by American Society of Hematology         Online ISSN: 1528-0020