Purification of Hageman factor (factor XII) on columns of popcorn- agarose
OD Ratnoff, B Everson, VH Donaldson and BH Mitchell
Purification of Hageman factor (HF, factor XII) from human plasma is a
tedious procedure and the product is not always in the precursor form.
Hojima has described a protein derived from corn kernels that inhibits the
enzymatic properties of HF. This inhibitor binds to the precursor form of
HF. Rapid purification of HF was achieved by using as the major
purification step adsorption of this clotting factor to popcorn inhibitor
bound to agarose. The product had a specific activity of 50.0 to 67.1
coagulant units of HF per milligram protein, and the yield was 33% to 40%
of the HF content of the starting plasma. The purified protein displayed a
single band upon unreduced or reduced sodium dodecyl sulfate polyacrylamide
gel electrophoresis and less than 0.1% was in an activated form, as
measured in coagulant assays. The technique described is more rapid and
reliable than methods described earlier.
Volume 67,
Issue 6,
pp. 1550-1553,
06/01/1986
Copyright © 1986 by The American Society of Hematology
