Carrier detection in hemophilia A: a cooperative international study. II.
The efficacy of a universal discriminant
PP Green, PM Mannucci, E Briet, R Ljung, CK Kasper, EM Essien, J Chediak, CR Rizza and JB Graham
Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by
eight laboratories on a total of 336 obligatory carriers of hemophilia A
and 137 normal women were used to answer several questions concerning the
construction of linear discriminants for carrier detection. It was found:
that a "universal" linear discriminant can be constructed which is suitable
for use in all laboratories and is nearly as effective as
laboratory-specific discriminants; that inclusion of age and ABO blood type
data improved the efficacy of these discriminants; that substitution of
alternative assays for F.VIII and VWF:Ag did not generally improve the
efficacy of the discriminants over that obtained using the bioassay for
F.VIII:C and Laurell's immunoassay for VWF:Ag; that linear discriminants
were far more effective than discriminants based on the F.VIII:C/VWF:Ag
ratio. A step-wise procedure is given which any laboratory may follow in
using the universal discriminant for carrier detection.
Volume 67,
Issue 6,
pp. 1560-1567,
06/01/1986
Copyright © 1986 by The American Society of Hematology
