Lymphocyte subsets in normal bone marrow
P Clark, DE Normansell, DJ Innes and CE Hess
Bone marrow aspirates and biopsies from ten normal donors were stained
directly with monoclonal antibodies specific for lymphocyte, monocyte, and
myeloid antigens, and were analyzed by flow cytometry. To avoid cell loss,
lymphocytes were not specifically isolated prior to staining. T cells
comprised 46% of aspirate lymphocytes and 22% of biopsy lymphocytes.
Further, the Leu-3:Leu-2 ratio of bone marrow T cells was below 1.0. B
cells comprised 8% to 11% of bone marrow lymphocytes in both aspirates and
biopsies, and there was a substantial percentage of cells in the lymphocyte
window that was negative for all B and T cell markers. The lymphocyte
window had very little myeloid contamination; however, when the myeloid
window was examined, staining was greater than 90%.
Volume 67,
Issue 6,
pp. 1600-1606,
06/01/1986
Copyright © 1986 by The American Society of Hematology
