Successful donor cell engraftment in a recipient of bone marrow from a
cadaveric donor
BR Blazar, LC Lasky, JP Perentesis, KV Watson, SE Steinberg, AH Filipovich, HT Orr and NK Ramsay
A 12-year-old male with acute lymphocytic leukemia received donor bone
marrow from his histocompatible father whose marrow was harvested 40
minutes postmortem after he suffered a myocardial infarction. The marrow
was stored in liquid nitrogen for 17 days prior to infusion into the
recipient. Trypan blue viability was greater than 99% for the fresh marrow.
Progenitor cell assays revealed that 20% of the CFU-MIX, 16% of the BFU-E,
10% of the CFU-E, and 17% of the CFU-GM were spared during the
cryopreservation period. Posttransplantation, the recipient had a leukocyte
count greater than 10(3)/microL by day 26. Southern blotting analysis
documented the donor origin of the peripheral blood mononuclear cells and
granulocytes isolated 46 days posttransplantation. Unfortunately, the
patient died of complications relating to graft-v-host disease 67 days
following transplantation. This case demonstrates the feasibility of
cadaveric marrow as a source of donor cells and is the first reported case
of documented leukocyte engraftment in a recipient of cadaveric marrow.
Volume 67,
Issue 6,
pp. 1655-1660,
06/01/1986
Copyright © 1986 by The American Society of Hematology
