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L Lu, LM Pelus, HE Broxmeyer, MA Moore, M Wachter, D Walker and E Platzer
The relationship between major histocompatibility complex class II antigens
(MHC class II, eg, HLA-DR, Ia), T lymphocytes, and the enhancement of
erythroid colony formation from BFU-E by prostaglandin E was analyzed using
normal bone marrow cells. In primary methylcellulose culture, the addition
of prostaglandin E1 (PGE1) to unseparated buffy coat, low-density, or
nonadherent low-density (NAL) marrow cells resulted in an enhancement of
the total number of erythroid (BFU-E) colonies observed. Treatment of bone
marrow cells with a monoclonal antihuman MHC class II antibody plus
complement (C') resulted in a reduction of the total number of colonies by
approximately 50% and abrogation of the enhancing effect of PGE1. Analysis
of accessory cell requirements by depletion of both adherent cells and
sheep erythrocyte rosetting lymphocytes (E+ cells) and reconstitution using
C' or anti- MHC class II antibody plus C'-treated T cell-depleted NAL
(NALT-) marrow cells and E+ cell populations treated with C' or anti-MHC
class II antibody plus C' demonstrated a requirement for MHC class II
antigen- T cells, but not adherent cells, and a requirement for MHC class
II antigen + BFU-E in order to observe the enhancing effect of PGE1 on
erythroid colony formation. Positive selection of BFU-E in NALT- bone
marrow expressing differing density distributions of MHC class II antigens
was accomplished with monoclonal anti-MHC class II antibodies and sorting
with a fluorescence-activated cell sorter (FACS). Addition of E+ cells to
the different populations of MHC class II antigen+ NALT- cells demonstrated
that the PGE-enhancing effects on erythroid colony formation were directly
related to increasing density distributions of MHC class II antigens on
BFU-E. Colony formation by BFU-E expressing a low density distribution of
MHC class II antigens or having no detectable MHC class II antigens, as
determined by FACS analysis, was not enhanced by PGE1 in the presence of
MHC class II antigen-positive or -negative T cells.
This article has been cited by other articles:
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| Copyright © 1986 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
