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Purified colony-stimulating factors enhance the survival of human
neutrophils and eosinophils in vitro: a rapid and sensitive microassay for
colony-stimulating factors
CG Begley, AF Lopez, NA Nicola, DJ Warren, MA Vadas, CJ Sanderson and D Metcalf
The survival of purified human blood neutrophils and eosinophils was
monitored using microwell cultures. Survival was enhanced in cultures
containing human or murine colony-stimulating factors (CSFs). The survival
of both cell types was enhanced by purified recombinant human
granulocyte-macrophage CSF and partially purified preparations of the
native molecule, CSF alpha. Neutrophil but not eosinophil survival was
enhanced by murine granulocyte-CSF and its human analogue CSF beta.
Eosinophil but not neutrophil survival was enhanced by murine eosinophil
differentiation factor (eosinophil CSF). The mature cell survival provided
an assay system for CSF that was 10(2) to 10(3) more sensitive than the
standard assay of stimulation of colony formation in agar cultures and
could be completed within 48 hours. These results demonstrated that CSF
induced and enhanced survival of mature human neutrophils and eosinophils
in vitro. Furthermore, the lineage specificity of purified murine CSFs was
retained in their action on human cells.
Volume 68,
Issue 1,
pp. 162-166,
07/01/1986
Copyright © 1986 by The American Society of Hematology
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