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JM Heard, B Sola, MA Martial, S Fichelson and S Gisselbrecht
The replication-competent Friend leukemia virus (F-MuLV) induces leukemias
involving three hematopoietic lineages after a latent period of several
months. In an attempt to elucidate the early events of the leukemogenic
process, we looked for a method allowing the isolation and the long term in
vitro maintenance of preleukemic cells. When established as long-term
cultures according to the technique described by Dexter et al, bone marrow
cells obtained from 7/7 apparently healthy F-MuLV-infected preleukemic mice
led to the accumulation of immature myeloblastic cells, and to the
generation of permanent myeloblastic cell lines, which in most cases
further became tumorigenic in preirradiated recipient animals. The delays
required to obtain cell lines were shorter when the duration of the in vivo
infection was longer, suggesting that these cells were committed into the
leukemogenic pathway before their transfer into culture flasks. The
myelomonocytic preleukemic cells exhibited normal sensitivity to purified
preparations of CSFs, but acquired the capacity to grow in the absence of
exogenous CSF stimulation. Examination of integrated provirus copies
demonstrated that the preleukemic cell proliferation involved a single or a
few clones which may progress in vitro from a preleukemic to a fully
malignant stage without major modifications of the integrated provirus
copies.
This article has been cited by other articles:
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| Copyright © 1986 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
