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PJ Krause, HL Malech, J Kristie, CM Kosciol, VC Herson, L Eisenfeld, WT Pastuszak, A Kraus and B Seligmann
We have used a mouse monoclonal antibody (31D8) to determine whether
differences in neutrophil (PMN) subpopulations might help explain decreased
PMN chemotaxis in neonates compared with that of adults. 31D8 has been
shown to bind heterogeneously to adult PMNs. Approximately 80% of the PMNs
that strongly bind 31D8 (31D8 "bright") are the same cells that depolarize
and migrate chemotactically when stimulated with the chemoattractant
N-formyl-methionylleucylphenylalanine, while the 20% that weakly bind 31D8
fail to similarly respond. All neonatal PMNs bound 31D8 heterogeneously.
There was a smaller population of 31D8 "bright" cells in neonates at birth
(76% +/- 6%, n = 45) compared with that of neonates at three to 15 days of
age (82% +/- 5%, n = 10, P less than 0.002) and both were smaller than that
of adults (88% +/- 4%, n = 45, P less than 0.001 and P less than 0.001).
Neonatal cord PMNs, which traversed a micropore filter in a modified Boyden
chemotaxis chamber in the presence of a chemoattractant, had an increased
percentage of 31D8 "bright" cells (89% +/- 7%) than did PMNs which remained
above the filter (82% +/- 7%, n = 10, P = 0.034). PMN chemotaxis was less
in neonates at birth (32.7 +/- 4.5 micron) than at three to six days of age
(36.8 +/- 11.3 micron) and both were decreased compared with that of adults
(69.1 +/- 12.4 micron, P less than 0.001 and P less than 0.001). These
findings indicate that decreased PMN chemotaxis in neonates may be due in
part to a smaller PMN subpopulation of highly motile cells.
This article has been cited by other articles:
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| Copyright © 1986 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
