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RD Brown, E Yuen, H Kronenberg and KA Rickard
Cord plasma contains colony-stimulating activity (CSA) which stimulates the
in vitro clonal growth of neutrophils, eosinophils, macrophages,
erythrocytes, and persisting mast cells in semisolid cultures. Analysis of
day 35 colonies in agar cultures was found to be a suitable means of
demonstrating this activity and discriminating between it and
granulocyte-macrophage colony-stimulating factor (GM-CSF). Serum (10%) from
patients with acute and chronic myeloid leukemia (AML and CML) was added to
normal human bone marrow cultures to search for similar activity in these
patient's serum. Although the number of colonies on day 12 (predominantly
neutrophils and macrophages) was not significantly different from the
number of colonies in cultures containing normal serum, the number of
colonies increased 500% in cultures containing CML serum on day 35. Serum
from patients with AML during regeneration also stimulated an increased
number of colonies on day 35. Although both eosinophil and mast cell
colonies were still present on day 35, only mast cell colonies persisted
for 150 days. On day 35, cultures containing 10% CML serum contained
predominantly eosinophil colonies (84%), whereas cultures containing AML
serum contained predominantly mast cell colonies (76%). Although serum
contains various CSFs, the specific factor which stimulates persisting mast
cell colonies may be the human equivalent of murine persisting (P)
cell-stimulating factor (Multi-CSF).
This article has been cited by other articles:
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| Copyright © 1986 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
