|
|
 Previous Article | Table of Contents | Next Article 
Fucose binding lectin for characterizing acute myeloid leukemia progenitor
cells
R Delwel, I Touw, F Bot and B Lowenberg
The reactivity of acute myeloid leukemia cells (AML) was determined in 29
patients using the fucose binding lectin Ulex europaeus agglutinin (UEA) as
surface marker. We show a marked heterogeneity in the UEA- binding
abilities of the cells in these patients as determined by fluorescence
analysis of the blasts labeled with the UEA coupled to the fluorescent
molecule FITC. The results suggest a correlation between the capability of
AML blast cells to bind UEA and cytologic maturation, because in 1 of 10
M1, 3 of 8 M2, 6 of 8 M4, and 1 of 3 M5 cytology types UEA binding to the
leukemic cells was apparent. In 13 cases, the cells gave rise to colonies
in vitro. The amount of UEA binding to AML colony-forming cells (AML-CFU)
was determined by cell sorting and subsequent colony culture of
UEA-negative, intermediately positive, and highly fluorescent cells.
AML-CFU from none of the four patients with M1 cytology were UEA positive,
whereas they showed intense reactivity with the lectin in 1 of 4 cases with
M2 cytology and in all 4 cases of M4. In these five cases with strongly UEA
positive AML-CFU, the fluorescence distribution of the colony formers
differed from that of the total leukemia population, indicating that
AML-CFU represent a subpopulation of AML cells with specific UEA-binding
properties. Normal bone marrow myeloid and multipotential colony-forming
cells (CFU-GM, CFU-GEMM) showed low or no binding of UEA. UEA-FITC appears
a useful reagent for membrane analysis of AML-CFU. In certain cases,
UEA-FITC labeling may be applied to discriminate AML-CFU from normal
hematopoietic progenitors.
Volume 68,
Issue 1,
pp. 41-45,
07/01/1986
Copyright © 1986 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Alberich Jorda, N. Rayman, M. Tas, S. E. Verbakel, N. Battista, K. van Lom, B. Lowenberg, M. Maccarrone, and R. Delwel
The peripheral cannabinoid receptor Cb2, frequently expressed on AML blasts, either induces a neutrophilic differentiation block or confers abnormal migration properties in a ligand-dependent manner
Blood,
July 15, 2004;
104(2):
526 - 534.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Barjesteh van Waalwijk van Doorn-Khosrovani, C. Erpelinck, W. L. J. van Putten, P. J. M. Valk, S. van der Poel-van de Luytgaarde, R. Hack, R. Slater, E. M. E. Smit, H. B. Beverloo, G. Verhoef, et al.
High EVI1 expression predicts poor survival in acute myeloid leukemia: a study of 319 de novo AML patients
Blood,
February 1, 2003;
101(3):
837 - 845.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. H. Hoefsloot, M. P. van Amelsvoort, L. C.A.M. Broeders, D. C. van der Plas, K. van Lom, H. Hoogerbrugge, I. P. Touw, and B. Lowenberg
Erythropoietin-Induced Activation of STAT5 Is Impaired in the Myelodysplastic Syndrome
Blood,
March 1, 1997;
89(5):
1690 - 1700.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
