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Effects of purified bacterially synthesized murine multi-CSF (IL-3) on
hematopoiesis in normal adult mice
D Metcalf, CG Begley, GR Johnson, NA Nicola, AF Lopez and DJ Williamson
Normal adult C57BL, BALB/c, and C3H/HeJ mice were injected
intraperitoneally three times daily for up to 6 days with 102,000 U (200
ng) per injection of purified, bacterially synthesized, Multipotential
colony-stimulating factor (CSF) (Interleukin-3) (rMulti- CSF) and compared
with control mice injected with serum/saline with or without added
endotoxin (1 ng/mL). Mice injected with rMulti-CSF exhibited tenfold rises
in blood eosinophil and twofold to threefold rises in neutrophil and
monocyte levels. The spleens from mice injected with rMulti-CSF showed a
50% increase in weight, elevated levels of maturing granulocytes,
eosinophils, nucleated erythroid cells and megakaryocytes, and up to
100-fold rises in mast cells. Progenitor cell frequencies in the spleen
were elevated sixfold to 18-fold. No significant changes were observed in
the marrow. Sixfold to 15-fold rises were observed in peritoneal cell
populations of mice injected with rMulti-CSF with evidence of increased
peritoneal macrophage phagocytic activity. Livers of C57BL mice, but not of
the other strains, exhibited increased numbers of infiltrating
hematopoietic cells whereas rises in mast cell numbers were observed in the
mesenteric lymph node, skin, and gut in BALB/c and C3H/HeJ mice. Endotoxin
was excluded as being responsible for the observed changes except possibly
those involving peritoneal macrophage phagocytic activity. The results
indicate that the injection of normal mice with rMulti-CSF significantly
stimulates the same types of hematopoietic populations as are stimulated in
vitro by Multi-CSF and indicate that this and other CSFs should be useful
in stimulating hematopoietic repopulation and functional activity in vivo.
Volume 68,
Issue 1,
pp. 46-57,
07/01/1986
Copyright © 1986 by The American Society of Hematology
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