Immunohistochemical localization of membrane and alpha-granule proteins in
plastic-embedded mouse bone marrow megakaryocytes and murine megakaryocyte
colonies
PE Stenberg, JH Beckstead, RP McEver and J Levin
Using an immunoperoxidase technique that permits optimal antigen
localization at the light microscope level, we have detected two platelet
alpha-granule constituents and three platelet membrane glycoproteins in
mouse bone marrow megakaryocytes and in murine megakaryocyte colonies grown
in soft agar culture for three to seven days. Using polyclonal antibodies
prepared against human platelet proteins, we have demonstrated labeling for
von Willebrand factor, fibrinogen, and the membrane glycoproteins IIIa and
GMP-140 in both bone marrow megakaryocytes and megakaryocyte colonies after
seven days of culture. Using monoclonal antibodies to membrane
glycoproteins IIb and GMP-140, we have demonstrated label in mouse bone
marrow megakaryocytes. Granulocyte and macrophage colonies were negative
for each of these markers. Murine bone marrow megakaryocytes and
megakaryocyte colonies demonstrated a similar enzyme histochemical pattern:
weakly positive for alpha-naphthyl acetate esterase and negative for
chloroacetate esterase. These data indicate that megakaryocytes grown in
soft agar culture express many of the same glycoproteins as bone marrow
megakaryocytes. Furthermore, the ability of antibodies directed against
human platelet membrane glycoproteins to identify murine megakaryocyte
glycoproteins indicates that these constituents have been highly conserved
during evolution.
Volume 68,
Issue 3,
pp. 696-702,
09/01/1986
Copyright © 1986 by The American Society of Hematology
