|
|
 Previous Article | Table of Contents | Next Article 
Expression of antigens of the platelet glycoprotein IIb/IIIa complex on
human hematopoietic stem cells
JK Fraser, MF Leahy and MV Berridge
Several cell surface and cytoplasmic markers specific for the
megakaryocyte-platelet lineage have been described. However, as yet, none
of these has been shown to be expressed on cells earlier than the committed
megakaryocyte progenitor, CFU-Meg. The present study was aimed at
determining whether platelet lineage antigens could be detected on human
pluripotential stem cells. Rabbit antiserum against human platelets (APS)
was extensively absorbed with erythrocytes and either platelets,
neutrophils, monocytes, or cells of the monocytic cell line U937. The
anti-stem cell antibodies in each absorbed antiserum were determined using
a complement-dependent cytotoxic assay for the pluripotential stem cell
CFU-mix. Platelets alone removed anti- stem cell antibodies from APS.
Absorption of APS with platelets from a patient with Glanzmann's
thrombasthenia failed to remove the anti-stem cell activity, providing
evidence for involvement of the platelet glycoprotein IIb/IIIa complex.
Antiserum against purified glycoprotein IIb and against glycoprotein IIIa
also recognized stem cells, resulting in reduced formation of mixed
colonies. Absorption of these antisera with normal platelets removed the
anti-stem cell activity, indicating that both IIb and IIIa are represented
on stem cells. Hence, cell surface antigens specific for the stem
cell-megakaryocyte-platelet pathway are expressed on the platelet
glycoprotein IIb/IIIa complex.
Volume 68,
Issue 3,
pp. 762-769,
09/01/1986
Copyright © 1986 by The American Society of Hematology
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. J. Ferkowicz, M. Starr, X. Xie, W. Li, S. A. Johnson, W. C. Shelley, P. R. Morrison, and M. C. Yoder
CD41 expression defines the onset of primitive and definitive hematopoiesis in the murine embryo
Development,
September 15, 2003;
130(18):
4393 - 4403.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Debili, C. Robin, V. Schiavon, R. Letestu, F. Pflumio, M.-T. Mitjavila-Garcia, L. Coulombel, and W. Vainchenker
Different expression of CD41 on human lymphoid and myeloid progenitors from adults and neonates
Blood,
April 1, 2001;
97(7):
2023 - 2030.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Jandrot-Perrus, S. Busfield, A.-H. Lagrue, X. Xiong, N. Debili, T. Chickering, J.-P. L. Couedic, A. Goodearl, B. Dussault, C. Fraser, et al.
Cloning, characterization, and functional studies of human and mouse glycoprotein VI: a platelet-specific collagen receptor from the immunoglobulin superfamily
Blood,
September 1, 2000;
96(5):
1798 - 1807.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. T.-L. Roux, V. Roullot, C. Poujol, T. Kortulewski, P. Nurden, and G. Marguerie
Thrombasthenic mice generated by replacement of the integrin alpha IIb gene: demonstration that transcriptional activation of this megakaryocytic locus precedes lineage commitment
Blood,
August 15, 2000;
96(4):
1399 - 1408.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Poullis and I. Malik
Thrombocytopenia and Glycoprotein IIb/IIIa Inhibitors: Causation or Association?
Circulation,
June 27, 2000;
101
(25):
e241 - e241.
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Gomez-Espuch, J. Corral, R. Gonzalez-Conejero, F. Ortuno, J.M. Moraleda, V. Vicente;, C. Ody, B. A. Imhof, and C. Corbel
Glycoprotein IIb/IIIa Expression on Hematopoietic Stem Cells: Constitutive Expression or Platelet Adhesion?
Blood,
November 1, 1999;
94(9):
3271 - 3273.
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Ody, P. Vaigot, P. Quere, B. A. Imhof, and C. Corbel
Glycoprotein IIb-IIIa Is Expressed on Avian Multilineage Hematopoietic Progenitor Cells
Blood,
May 1, 1999;
93(9):
2898 - 2906.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Poujol, D. T.-L. Roux, P. Tropel, V. Roullot, A. Nurden, G. Marguerie, and P. Nurden
Ultrastructural Analysis of Bone Marrow Hematopoiesis in Mice Transgenic for the Thymidine Kinase Gene Driven by the alpha IIb Promoter
Blood,
September 15, 1998;
92(6):
2012 - 2023.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Tropel, V. Roullot, M. Vernet, C. Poujol, H. Pointu, P. Nurden, G. Marguerie, and D. Tronik-Le Roux
A 2.7-kb Portion of the 5' Flanking Region of the Murine Glycoprotein alpha IIb Gene Is Transcriptionally Active in Primitive Hematopoietic Progenitor Cells
Blood,
October 15, 1997;
90(8):
2995 - 3004.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
