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Thymic regulation of hematopoiesis. III: Isolation of helper and suppressor
populations using counterflow centrifugal elutriation
SJ Sharkis, C Cremo, MI Collector, SJ Noga and AD Donnenberg
We have evidence that thymic regulatory cells can either enhance or inhibit
the growth of hematopoietic progenitors in vitro. We have suggested that
two separate populations are responsible for this regulatory interaction
but isolation of the cell types has proven difficult. We now report the
isolation by counterflow centrifugal elutriation (CCE) of two separate
populations of thymocytes which regulate erythropoiesis in coculture. We
demonstrate that a minority population (less than 10%) of slow sedimenting
elutriated thymocytes provide a helper function whereas the suppressor
population is the majority population. Furthermore, some thymocytes of
intermediate cell volume neither enhance nor inhibit erythroid colony
growth. We conclude that isolation of thymic subsets can lead to
identification of populations which induce cell-cell regulation of
hematopoietic progenitors resulting in both a positive and negative
feedback control of growth.
Volume 68,
Issue 3,
pp. 787-789,
09/01/1986
Copyright © 1986 by The American Society of Hematology
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