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Characterization of host cells involved in resistance to marrow grafts in
dogs transplanted from unrelated DLA-nonidentical donors
RF Raff, HJ Deeg, TP Loughran , TC Graham, JA Aprile, GE Sale and R Storb
A canine model of marrow transplantation was used to further define the
host cells mediating resistance to marrow engraftment. Recipient dogs were
given 9.2 Gy of total body irradiation followed by marrow infusion from
unrelated DLA-nonidentical donors. No postgrafting immunosuppression was
given. At three and ten days posttransplantation recipient marrow and
peripheral blood cells were obtained and characterized by the following in
vitro studies: morphologic analysis; phenotypic analysis with monoclonal
antibodies; assays for natural killer cell (NK) activity; and cocultures
with donor marrow to study the effect on donor CFU-GM growth. Daily
differential cell counts revealed a proliferation of peripheral blood
mononuclear cells approximately eight days posttransplant. By day 10
surviving host cells were uniformly large granular lymphocytes which were
phenotypically of T cell lineage, had NK activity, and were capable of
suppressing donor marrow CFU-GM growth. Mononuclear cells from dogs given
total body irradiation only and no marrow infusion (radiation control
group), did not suppress CFU-GM growth when cocultured with marrow from
unrelated DLA-mismatched dogs. These results suggest that radioresistant
host cells with the morphology of large granular lymphocytes and NK
activity and which proliferate in response to the infused donor marrow
cells mediate resistance to DLA-nonidentical marrow grafts. It remains to
be determined, however, whether in vitro functional studies reflect the
mechanisms involved in vivo.
Volume 68,
Issue 4,
pp. 861-868,
10/01/1986
Copyright © 1986 by The American Society of Hematology
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