Enhanced recovery of gut-associated lymphoid tissue by infusion of buffy
coat cells and gut leukocytes in the murine syngeneic bone marrow
transplantation model
GS Leventon, SS Kulkarni, RA White and AR Zander
Ways of accelerating recovery of the mucous membrane immune system in
lethally irradiated mice following syngeneic bone marrow transplantation
were studied over a 35-day period by quantification of jejunal
intraepithelial lymphocytes (IELs) and lamina propria plasma cells.
Recovery after a low bone marrow dose allowing 100% animal survival (LBM)
was compared with a high (five times minimal) dose (HBM), or a minimal dose
augmented with equal numbers of buffy coat cells (LBM + BC) or small gut
mucosal lymphocytes (LBM + GL). The maximal decline and subsequent peak
repopulation of IELs were: LBM, days 7 through 14, peaking suboptimally by
day 28; HBM, day 14, peaking suboptimally but higher than LBM by day 35;
LBM + BC, days 11 through 14, peaking at control levels by day 35; and LBM
+ GL, day 7, peaking at control levels by day 28. The IEL decline was most
severe with LBM and HBM treatment and least with LBM + GL. All transplant
groups experienced maximal plasma cell decline by day 7. LBM had the most
severe depletion, and LBM + GL had the least. Recovery to control levels
for the LBM, HBM, LBM + BC and LBM + GL groups occurred by days 28, 21, 21,
and 14, respectively. In all instances, greater than 95% of the plasma
cells were IgA positive.
Volume 68,
Issue 5,
pp. 1003-1007,
11/01/1986
Copyright © 1986 by The American Society of Hematology
