Lymphokine(s) from isolated T lymphocyte subpopulations support
multilineage hematopoietic colony and megakaryocytic colony formation
L Kanz, GW Lohr and AA Fauser
Conditioned medium derived from peripheral mononuclear low-density cells
stimulated with phytohemagglutinin (PHA) supports the growth of
noncommitted hematopoietic progenitors from marrow and peripheral blood
cells. These immature progenitors (CFU-GEMM) can be identified in culture
as multilineage hematopoietic colonies containing erythroblasts,
eosinophilic, basophilic and neutrophilic granulocytes, megakaryocytes,
macrophages, and T and B lymphocytes. In this report, we describe the
effect of lymphokines released from purified T lymphocyte preparations of
helper (T4) and suppressor/cytotoxic (T8) phenotype derived from peripheral
blood on the growth of multilineage hematopoietic colonies and
megakaryocytic colonies. It was found that PHA-stimulated lymphocytes of T4
phenotype and, to a lesser extent, of T8 phenotype elaborate lymphokine(s)
that support the growth and development of multilineage colonies
(CFU-GEMM), granulopoietic colonies (CFU-C), erythroid bursts (BFU-E) and
megakaryocytic colonies (CFU-M) by nonadherent and T cell-depleted bone
marrow cells.
Volume 68,
Issue 5,
pp. 991-995,
11/01/1986
Copyright © 1986 by The American Society of Hematology
