Lysis of human fibroblast colony-forming cells and endothelial cells by
monoclonal antibody (6-19) and complement
CN Abboud, RE Duerst, CN Frantz, DH Ryan, JL Liesveld and JK Brennan
The murine IgG2a monoclonal antibody 6-19 binds to a wide variety of
nonhematopoietic cells including human marrow-derived stromal cells but
does not bind to marrow or peripheral blood cells. We studied the effects
of this antibody and rabbit complement on marrow cells. Fibroblast colonies
were eliminated from light density marrow cells by a single incubation with
monoclonal antibody 6-19 and complement. The growth and composition of
granulocytic and erythroid colonies were unaffected. Specific complement
mediated cytotoxicity of the antibody was confirmed on passaged human
fibroblasts derived from marrow (more than 99.6% of fibroblasts are killed
by a single treatment). Similar results were obtained with human umbilical
cord endothelial cells. In addition, such treatment abolished the
initiation of Dexter culture stroma. Incubation of bone marrow cell
suspensions with this antibody and complement will allow the study of
stroma-free marrow cells in long- term liquid cultures.
Volume 68,
Issue 6,
pp. 1196-1200,
12/01/1986
Copyright © 1986 by The American Society of Hematology
