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WE Fibbe, J van Damme, A Billiau, PJ Voogt, N Duinkerken, PM Kluck and JH Falkenburg
An electrophoretically pure preparation of natural human interleukin-1
(IL-1) was shown to stimulate in vitro colony formation in human bone
marrow cultures. Day 4 myeloid cluster-forming cells (CFC), as well as
early (day 7) and late (day 10) granulocyte-macrophage colony-forming units
(CFU-GM) were stimulated in a dose-dependent fashion. At optimal
concentrations of IL-1, the number of day 4 CFC reached 72%, the number of
day 7 CFU-GM reached 32%, and the number of day 10 CFU-GM reached 80% of
the respective numbers of colonies obtained by addition of crude
leukocyte-conditioned medium (LCM). The IL-1-induced stimulatory effect on
CFU-GM growth could be completely neutralized by a rabbit anti-IL-1
antiserum. Colony growth was abrogated by depleting the marrow cell
suspensions of phagocytic cells prior to IL-1 addition. Conversely, the
effect could be reintroduced by addition of marrow-derived adherent cells
to bone marrow cell suspensions that had been depleted of both phagocytic
and E rosetting T cells. Furthermore, media conditioned by bone
marrow-derived adherent cells or by peripheral blood mononuclear phagocytes
in the presence but not in the absence of IL-1, stimulated in vitro colony
growth of phagocyte-depleted bone marrow cell suspensions. These results
indicate that IL-1 induces release of granulocyte-macrophage
colony-stimulating activity (GM-CSA) from human mononuclear phagocytes.
This article has been cited by other articles:
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| Copyright © 1986 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
