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Stromal cells in myeloid and lymphoid long-term bone marrow cultures can
support multiple hemopoietic lineages and modulate their production of
hemopoietic growth factors
A Johnson and K Dorshkind
Hemopoiesis in long-term bone marrow cultures (LTBMC) is dependent on
adherent stromal cells that form an in vitro hemopoietic microenvironment.
Myeloid bone marrow cultures (MBMC) are optimal for myelopoiesis, while
lymphoid bone marrow cultures (LBMC) only support B lymphopoiesis. The
experiments reported here have made a comparative analysis of the two
cultures to determine whether the stromal cells that establish in vitro are
restricted to the support of myelopoiesis or lymphopoiesis, respectively,
and to examine how the different culture conditions affect stromal cell
physiology. In order to facilitate this analysis, purified populations of
MBMC and LBMC stroma were prepared by treating the LTBMC with the
antibiotic mycophenolic acid; this results in the elimination of
hemopoietic cells while retaining purified populations of functional
stroma. Stromal cell cultures prepared and maintained under MBMC conditions
secreted myeloid growth factors that stimulated the growth of
granulocyte-macrophage colonies, while no such activity was detected from
purified LBMC stromal cultures. However, this was not due to the inability
of LBMC stroma to mediate this function. Transfer of LBMC stromal cultures
to MBMC conditions resulted in an induction of myeloid growth factor
secretion. When seeded under these conditions with stromal cell- depleted
populations of hemopoietic cells, obtained by passing marrow through nylon
wool columns, the LBMC stromal cells could support long- term myelopoiesis.
Conversely, transfer of MBMC stroma to LBMC conditions resulted in a
cessation of myeloid growth factor secretion; on seeding these cultures
with nylon wool-passed marrow, B lymphopoiesis, but not myelopoiesis,
initiated. These findings indicate that the stroma in the different LTBMC
are not restricted in their hemopoietic support capacity but are sensitive
to culture conditions in a manner that may affect the type of
microenvironment formed.
Volume 68,
Issue 6,
pp. 1348-1354,
12/01/1986
Copyright © 1986 by The American Society of Hematology
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