Hyposialylation of differentiation-inducer-resistant HL-60 cells
RE Gallagher, DA Giangiulio, CS Chang, CJ Glover and RL Felsted
The total sialic acid concent of retinoic acid (RA)-resistant or 6-
thioguanine (6TG)-resistant HL-60 cells was more than tenfold lower and of
dimethylsulfoxide (DMSO)-resistant HL-60 cells was approximately twofold
lower than that of parental, wild-type (wt) HL-60 cells.
Neuraminidase-inaccessible, ie residual cell-associated sialic acid after
neuraminidase treatment, was four- to twelvefold lower in the three
differentiation-inducer-resistant sublines than in the parent line.
Neuraminidase treatment of 125I-labeled surface membrane glycoproteins
(SMGs) from wt HL-60 cells converted the two-dimensional gel
electrophoretic pattern to one having features in common with RA- and
6TG-resistant cells. However, neuraminidase treatment did not alter the
sensitivity of wt HL-60 cells to differentiation induction by RA,
hypoxanthine (purine base), or DMSO. These results indicate that
differences in peripheral, neuraminidase-accessible sialic acids are
important determinants of the gel electrophoretic mobility of the SMGs of
the HL-60 line and sublines but are not likely related to the
differentiation-resistance mechanism. Further studies are required to
determine if hyposialylation of cryptic, neuraminidase-inaccessible sites
has functional significance.
Volume 68,
Issue 6,
pp. 1402-1406,
12/01/1986
Copyright © 1986 by The American Society of Hematology
