SEARCH:   Advanced

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chitambar, C. R. Articles by Zivkovic, Z. Search for Related Content PubMed PubMed Citation Articles by Chitambar, C. R. Articles by Zivkovic, Z. Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Inhibition of hemoglobin production by transferrin-gallium CR Chitambar and Z Zivkovic Recent clinical trials evaluating gallium nitrate as a chemotherapeutic agent have reported the development of microcytic hypochromic anemia in patients treated with this agent. Because gallium is known to bind avidly to transferrin, we examined the effect of transferrin-gallium (Tf-Ga) on hemoglobin production by Friend erythroleukemia cells in vitro. Cellular hemoglobin production, as assessed by benzidine staining, cellular hemoglobin content, and 59Fe incorporation into heme, was significantly decreased following exposure of cells to Tf-Ga. Tf-Ga led to an early decrease in cellular 59Fe incorporation even before changes in hemoglobin production were detected. A marked increase in cellular transferrin receptor expression occurred following exposure of cells to Tf-Ga. Tf-Ga inhibition of hemoglobin production could be reversed and hemoglobin production could be restored to normal by addition to the media of either transferrin-iron (Tf-Fe) or iron- pyridoxal isonicotinoyl hydrazone, a compound capable of supplying iron directly to reticulocytes for heme synthesis without transferrin as a mediator. These studies provide an explanation for the development of anemia in patients treated with gallium nitrate and suggest that gallium's mechanism of chemotherapeutic action includes inhibition of cellular iron incorporation. Volume 69, Issue 1, pp. 144-149, 01/01/1987 Copyright © 1987 by The American Society of Hematology CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: O. Olakanmi, J. B. Stokes, S. Pathan, and B. E. Britigan Polyvalent Cationic Metals Induce the Rate of Transferrin-independent Iron Acquisition by HL-60 Cells J. Biol. Chem., January 31, 1997; 272(5): 2599 - 2606. [Abstract] [Full Text] [PDF]

	Copyright © 1987 by American Society of Hematology         Online ISSN: 1528-0020