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Thrombocytopoietic response to immunothrombocytopenia in nude mice
S Ebbe, J Levin, K Miller, T Yee, F Levin and E Phalen
Thrombocytopoiesis was evaluated in T cell-deficient nu/nu mice and in T
cell-replete nu/+ controls to determine if abnormalities would be
associated with the deficiency of T cells. Mice were studied in the
unperturbed steady state and after acute immunothrombocytopenia was induced
by an injection of guinea pig antimouse platelet serum (APS). The state of
thrombocytopoiesis was determined from platelet counts, megakaryocyte size,
megakaryocyte number, and numbers of Meg-CFC. Splenic lymphocytes were
evaluated by response to the mitogens bacterial lipopolysaccharide (LPS),
phytohemagglutinin (PHA), and concanavalin A (Con A). Hematocrits,
reticulocyte counts, leukocyte counts, marrow cellularity, GM-CFC, and
BFU-E also were measured. Steady state thrombocytopoiesis was identical in
nu/nu and nu/+ mice. In response to an injection of APS, acute
thrombocytopenia was followed by macromegakaryocytosis and rebound
thrombocytosis in mice of both genotypes. Splenic Meg-CFC increased in nude
mice after APS or an injection of normal guinea pig serum (NGpS), and
splenic GM-CFC increased after APS. Neither Meg-CFC nor GM-CFC increased in
the spleens of nu/+ mice, but they showed early transient increases in bone
marrow that did not occur in nu/nu mice. Sporadic, but weak, mitogenic
responses to PHA or Con A were occasionally observed with nu/nu spleen
cells, but these did not correlate with the state of thrombocytopoiesis.
The results demonstrated that platelet production was normal in nu/nu mice
and that megakaryocytopoiesis and platelet production responded to the
stimulus imposed by acute immunothrombocytopenia. Increases in
megakaryocyte size and platelet production occurred independently of
changes in numbers of Meg-CFC, GM- CFC, or BFU-E. A normal complement of T
cells appears to be unnecessary for normal platelet production and its
augmentation in response to the stimulus of acute immunothrombocytopenia in
vivo.
Volume 69,
Issue 1,
pp. 192-198,
01/01/1987
Copyright © 1987 by The American Society of Hematology
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