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JR Zucali, HE Broxmeyer, CA Dinarello, MA Gross and RS Weiner
Stimulators of human erythroid burst-forming units (BFU-E) and
multipotential colony-forming cells (CFU-GEMM) can be produced by a number
of different cell types. A product of human peripheral blood monocytes,
interleukin 1 (IL-1), was evaluated for its ability to stimulate fibroblast
cultures to produce stimulators of human bone marrow BFU-E and CFU-GEMM
colony formation. BFU-E and CFU-GEMM colony formation was evaluated using
low-density, nonadherent low-density, and T lymphocyte-depleted nonadherent
low-density human bone marrow cells cultured in the presence of a source of
pure human erythropoietin. Both human monocyte conditioned medium (MCM) and
human recombinant IL-1 (hrIL-1) induced fibroblasts to produce stimulators
of BFU-E and CFU- GEMM in a dose-dependent fashion with maximal colony
formation occurring when fibroblasts were stimulated by 25% MCM or 140
ng/mL ROO6B hrIL-1, or 1.25 to 5 ng/mL ROO6T hrIL-1. Preincubation of MCM
and hrIL-1 with an antibody to IL-1 inactivated the ability of MCM and
hrIL- 1 to induce the release of erythroid and multipotential colony
stimulating activity from fibroblasts. These results suggest that
monocyte-derived IL-1 is involved in regulating the production of humoral
stimulators of early human hematopoietic progenitors.
This article has been cited by other articles:
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| Copyright © 1987 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
