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SP Ackland, CA Westbrook, MO Diaz, MM Le Beau and JD Rowley
The concept of lineage fidelity in acute leukemia has recently been
challenged by the finding of rearrangements of the immunoglobulin heavy
chain genes in a leukemic cell line and in a small number of sporadic cases
of acute nonlymphocytic leukemia with a monocytic phenotype. We therefore
screened leukemic blood or bone marrow samples of 33 adult patients with
acute nonlymphocytic leukemia of FAB types M4 (23 patients) and M5 (10
patients); 28 were obtained at diagnosis and 5 at relapse. All cases were
well characterized pathologically and histochemically. Cytogenetic analysis
performed in each case demonstrated karyotypes that were representative of
those generally seen in these types of leukemia, with a clonal abnormality
present in all except 9 of 32 patients who were successfully studied. DNA
prepared from each sample was digested with the restriction enzyme BamH1
and analyzed by Southern blot hybridization to probes for the JH region of
the immunoglobulin heavy chain. All 33 cases had DNA retained in the
germline configuration with no evidence of rearrangement. This finding
supports the concept of lineage fidelity, and suggests that true
interlineage infidelity, myeloid to lymphoid, is a rare occurrence in adult
acute nonlymphocytic leukemia.
This article has been cited by other articles:
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| Copyright © 1987 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
