A monoclonal antibody detecting a novel antigen expressed in the HTLV-I-
infected cells
F Tsubai, Y Namba, M Kohno, S Hanada, M Matsumoto, M Shamoto and M Hanaoka
A monoclonal antibody, FTF 148, was prepared by hybridizing murine myelomal
cells (NS-1) and spleen cells of BALB/c mice immunized with cultured cells
derived from an adult T cell leukemia (ATL) patient (KUT- 2 cells). This
monoclonal antibody reacted with all of the human T cell leukemia virus I
(HTLV-I)-infected cell lines tested but did not react with other T cell
lines derived from acute lymphocytic leukemia, Epstein-Barr
virus-transformed B cell lines, or an erythroleukemic cell line. This
monoclonal antibody was not directed to viral antigens because it reacted
equally well with almost all KUT-2 and MT-1 cells, only 1% to 3% of which
were ATL-associated antigen-positive. In contrast to interleukin 2
receptors expressed on both ATL cells and normal
phytohemagglutinin-stimulated blasts, this antigen was not expressed on the
latter cells. The antigen, mainly expressed on the cell membrane, was
analyzed by metabolic labeling with 3H-leucine and surface labeling with
125I followed by cell lysis and immunoprecipitation with the FTF 148
antibody. The findings obtained by sodium dodecyl sulfate-polyacrylamide
gel electrophoresis revealed that p50 and p74 proteins were specifically
precipitated and the antigen was also different from the product of the Xs
gene of HTLV-I.
Volume 69,
Issue 2,
pp. 430-436,
02/01/1987
Copyright © 1987 by The American Society of Hematology
