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Purification and partial amino acid sequence of human platelet membrane glycoproteins IIb and IIIa

A Hiraiwa, A Matsukage, H Shiku, T Takahashi, K Naito and K Yamada

The glycoprotein (GP) IIb-IIIa complex was isolated from human platelet membranes by immunoaffinity chromatography using a monoclonal antibody specific for GP IIb-IIIa. GP IIb and IIIa were further separated in the presence of sodium dodecyl sulfate (SDS) by gel filtration high- performance liquid chromatography (HPLC). Two cycles of this procedure yielded almost complete separation of homogeneous preparations of GP IIb and IIIa. Each protein was then digested with lysyl endopeptidase (Achromobacter protease I), which cleaves at the carboxyl side of lysine residues, and the resulting oligopeptides from GP IIb and IIIa were fractionated with HPLC using a C18 reverse-phase column. Comparison of the elution profiles showed no obvious homology between the two proteins. Amino acid sequences of selected oligopeptides from each glycoprotein were determined using a gas-phase protein sequencer. Sixty amino acid residues (26 residues for IIb and 34 residues for IIIa) were identified.

Volume 69, Issue 2, pp. 560-564, 02/01/1987
Copyright © 1987 by The American Society of Hematology


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  Copyright © 1987 by American Society of Hematology         Online ISSN: 1528-0020