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K Kon, N Maeda and T Shiga
The effect of shear force (depending on shear rate and viscosity of
extracellular medium) and hematocrit of RBC suspension on RBC deformation
was studied quantitatively using a cone-plate rheoscope with various kinds
of cells, ie, partially hemolyzed (PH) cells, density-fractionated intact
cells, and diamide-treated cells. The deformation index (DI) of
ellipsoidally deformed cells was shown to be a function of beta gamma eta
ex(eta ex/eta in)alpha, where gamma eta ex is applied shear stress, eta ex
and eta in are external and internal viscosities, respectively, and alpha
and beta are adjustable parameters related to the membrane viscoelastic
properties. The increase of suspension viscosity at higher hematocrits
(Hts) generally enhanced the ellipsoidal deformation of cells, in the same
manner as increasing the suspending medium viscosity of a diluted cell
suspension. The suppressing effect on cell deformation appeared above a
certain Ht. When intact cells were mixed with glutaraldehyde-treated,
hardened cells, the ellipsoidal deformation of intact cells was disturbed.
The suppression of deformation probably occurred through disturbance of
laminar flow-lines around intact cells.
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| Copyright © 1987 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
