Factor XIII A is synthesized and expressed on the surface of U937 cells and
alveolar macrophages
RL Kradin, GW Lynch, JT Kurnick, M Erikson, RB Colvin and J McDonagh
Factor XIII A subunit was detected in U937 cells and human alveolar
macrophages by immunohistology and Western blotting. U937 cells synthesize
factor XIII A subunit de novo under serum-free, platelet- free conditions,
as indicated by 35S-methionine labeling and immunoprecipitation.
Thrombin-dependent activity was demonstrated to account for 98% of the
total transglutaminase activity in U937 cells (1.5 micrograms per 0.5 X
10(6) cells/mL). Intact U937 cells and alveolar macrophages and homogenates
from these cells cross-linked fibrin to form gamma-gamma and
alpha-polymers. Factor XIII A was detected on the surface of intact U937
cells and macrophages by flow cytometry and 125I-labeling and
immunoprecipitation. Cell surface expression of factor XIII A was augmented
in the presence of several soluble macrophage activators; however, no
concurrent increase in its biosynthesis was observed. The presence and cell
surface expression of factor XIII A subunit within macrophages suggest new
pathways by which these cells may function in clotting and in the
remodeling of the extracellular matrix during inflammation and wound
healing.
Volume 69,
Issue 3,
pp. 778-785,
03/01/1987
Copyright © 1987 by The American Society of Hematology
