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G Pianezze, I Gentilini, M Casini, P Fabris and P Coser
It is generally assumed that chronic lymphocytic leukemia of B cell origin
(B-CLL) is characterized by the presence of surface membrane
immunoglobulins (SmIg) and by the absence of cytoplasmic immunoglobulins
(CyIg). In a variable number of cases SmIg are not detectable because of
their low density on the cellular surface. Because a constant presence of
CyIg in 20 subjects suffering from B-CLL has been reported recently, we
reexamined 15 SmIg-negative and 10 SmIg- positive B-CLL patients by SmIg
and CyIg determinations. We used a direct immunofluorescence method on
peripheral blood mononuclear cells for the detection of SmIg and, after
fixation, for CyIg. CyIg were detectable in 24 out of 25 cases, with a
fluorescence intensity ranging from weak to moderate. The existence of
frequent negative results for CyIg determination in B-CLL reported in the
literature probably depends on the low sensitivity of the method used. We
conclude that CyIg determination is useful in phenotyping every B-CLL
patient, especially SmIg-negative ones.
This article has been cited by other articles:
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| Copyright © 1987 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
