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Erythropoietic repopulating ability of stem cells from long-term marrow
culture
DE Harrison, CP Lerner and E Spooncer
Hemopoietic precursors are heterogeneous with respect to their capacity for
self-renewal and long-term repopulating ability. Bone marrow cultures
produce a variety of precursors over many weeks, including CFU- S; however,
it is important to determine whether these populations retain the
functional ability shown by fresh marrow. The most primitive precursor or
stem cells have the most long-term repopulating ability. We here describe
direct measurements of this ability in cells from marrow cultures by using
competitive repopulation assays. Cultured adherent cells repeatedly showed
less capacity than fresh marrow cells to repopulate erythropoiesis in
irradiated recipients, whereas cultured suspension cells consistently had
less capacity than adherent cells. Concentrations of macroscopic CFU-S
measured at nine or 12 days were similar in cultured adherent and
suspension cells and generally lower than those in fresh marrow. In every
experiment, the long-term repopulating ability of the marrow cells used was
substantially reduced after transfer into tissue culture. Thus, primitive
stem cells may not proliferate in such cultures despite extensive
production of CFU-S and more differentiated cell types.
Volume 69,
Issue 4,
pp. 1021-1025,
04/01/1987
Copyright © 1987 by The American Society of Hematology
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