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The in vitro synthesis of polypeptides for the platelet membrane
glycoproteins IIb and IIIa
SM Silver, MM McDonough, G Vilaire and JS Bennett
The platelet membrane glycoproteins IIb (GpIIb) and GpIIIa form calcium-
dependent heterodimers containing binding sites for fibrinogen, von
Willebrand factor, and fibronectin. Although GpIIb and GpIIIa are distinct
proteins, both GpIIb and GpIIIa are deficient in platelets from individuals
with the recessive disorder Glanzmann's thrombasthenia. To gain a better
understanding of the genetic basis for GpIIb and GpIIIa synthesis, we
studied their synthesis by two human leukemia cell lines, HEL and K562. HEL
cells contained complexes of GpIIb and GpIIIa, and K562 cells expressed
GpIIIa, but not GpIIb, when stimulated with phorbol-12-myristate-13-acetate
(PMA). RNA from HEL cells directed the in vitro synthesis of a 110,000-Mr
precursor for GpIIb and a 92,000-Mr precursor for GpIIIa, which indicates
that the synthesis of GpIIb and GpIIIa by HEL cells is directed by separate
mRNAs. In contrast, RNA from PMA-stimulated K562 cells only directed the
synthesis of a 92,000-Mr precursor for GpIIIa. The dissociation of GpIIb
and GpIIIa synthesis in K562 cells suggests that GpIIb and GpIIIa may be
the products of separate genes.
Volume 69,
Issue 4,
pp. 1031-1037,
04/01/1987
Copyright © 1987 by The American Society of Hematology
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