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Megakaryocyte morphogenesis stimulated in vitro by whole and partially
fractionated thrombocytopenic plasma: a model system for the study of
platelet formation
RM Leven and MK Yee
Isolated guinea pig megakaryocytes were cultured in the presence of plasma
from normal or thrombocytopenic rabbits. Thrombocytopenic but not normal
plasma stimulated formation of long cytoplasmic processes and cytoplasmic
fragmentation. Activity was found in the 60% to 80% ammonium sulfate
fraction of thrombocytopenic plasma but not in the 0% to 60% fraction. The
60% to 80% fraction of normal plasma contained a small amount of activity.
Both colchicine and vincristine inhibited the morphogenesis stimulated by
thrombocytopenic plasma. Cytochalasin B and D both mimicked the
thrombocytopenic plasma-induced morphological change and affected more
megakaryocytes than did the thrombocytopenic plasma. Cytochalasin and
thrombocytopenic plasma together had a synergistic effect, causing many
megakaryocytes to form processes and break into cytoplasmic fragments 3 to
6 microns in diameter. Immunofluorescence staining with antitubulin
antiserum showed that cytoplasmic processes formed in the presence of
thrombocytopenic plasma contain microtubules and that fragments released by
the megakaryocytes contain microtubule rings. A model for the cytoskeletal
basis of platelet formation is proposed.
Volume 69,
Issue 4,
pp. 1046-1052,
04/01/1987
Copyright © 1987 by The American Society of Hematology
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